Table 1: Oligos used in this study.


Oligos Sequence

 mRFPF  5′-CCACCGGAGCTTACCATGGCCAGCTCCGAGGATG-3′

 mRFPR  5′-CTTGTACTCGGTCATAGGGCCGGGATTCTCCT-3′

 VectorF  5′-ATGACCGAGTACAAGCCCACG-3′

 VectorR  5′-GGTAAGCTCCGGTGGATCC-3′

 sgRNAF  5′-CCGGGCAGCCAGCCTAGTGGGAGC-3′

 sgRNAR  5′-AAACGCTCCCAGCTAGGCTGGCTGC-3′

 RFLPtempF  5′-GTAGCTGAGGCTGGCCTTAAG-3′

 RFLPtempR  5′-CACGTACTGTGTGACCTATGG-3′