Table 1: Oligos used in this study.
Oligos | Sequence |
mRFPF | 5′-CCACCGGAGCTTACCATGGCCAGCTCCGAGGATG-3′ |
mRFPR | 5′-CTTGTACTCGGTCATAGGGCCGGGATTCTCCT-3′ |
VectorF | 5′-ATGACCGAGTACAAGCCCACG-3′ |
VectorR | 5′-GGTAAGCTCCGGTGGATCC-3′ |
sgRNAF | 5′-CCGGGCAGCCAGCCTAGTGGGAGC-3′ |
sgRNAR | 5′-AAACGCTCCCAGCTAGGCTGGCTGC-3′ |
RFLPtempF | 5′-GTAGCTGAGGCTGGCCTTAAG-3′ |
RFLPtempR | 5′-CACGTACTGTGTGACCTATGG-3′ |