Generating a New sgRNA Vector, pGL3-U6-sgRNA-PGK-mRFP-T2A-PuroR, to Improve Base Editing: Figure 4

Figure 4: RFLP analysis of Munc13-1^null clones. (a) Schematic diagram of NheI-based RFLP analysis of potential Munc13-1^null clones. (b) A 799 bp Munc13-1 DNA fragment containing the editing site was amplified from individual clones using One-Taq DNA polymerase. Following NheI digestion and subsequent agarose (1.5%) gel electrophoresis, monoallelic mutants were distinguished from the WT.