Figure 3: Use of pGL3-U6-sgRNA-PGK-mRFP-T2A-PuroR in base editing. RBL-2H3 cells were transfected with pGL3-U6-sgRNAMunc13-1-PGK-mRFP-T2A-PuroR and pCMV_BE4max, and grown in complete media with puromycin. Fluorescence microscopy shows that virtually all puromycin resistant cells expressed red fluorescent signals (a) and (b). In contrast, RBL-2H3 cells that lacked pGL3-U6-sgRNAMunc13-1-PGK-mRFP-T2A-PuroR did not display red fluorescent signals (c) and (d). Scale bar, 100 μm.