Figure 1: New sgRNA vector pGL3-U6-sgRNA-PGK-mRFP-T2A-PuroR. (a) Schematic representation of the new plasmid. (b) DNA sequence of the region between the PGK promoter and the puromycin resistance gene. The mRFP cDNA (without the stop codon) is in frame with the T2A linker and the puromycin resistance gene. The entire construct has been sequence verified. The dotted lines represent codons not depicted in the diagram.