Figure 3: Effect of cryopreservation of ovarian tissue on primordial follicle morphology. Proportional ORs and 95% CIs of follicles being graded as ≥ 1 in fresh tissue compared to cryopreserved-thawed tissue. Ovine ovarian tissue was processed immediately and either fixed or cryopreserved-thawed, and fixed (n = 6) in Bouin’s solution. Primordial follicles (oocyte surrounded by a single layer of flattened pregranulosa cells) were analysed using H&E staining and scored based on the presence of molecular markers (dense chromatin, eosinophilia, shrunken ooplasm [one point each] or pyknosis [two points]) as normal (0), affected (1), degenerating (2) or atretic (≥ 3). Black CI bars indicate P < .05.