Figure 1: Health grading of primordial follicles based on morphological appearance. Bouin’s-fixed ovine ovarian tissue was analysed for the presence of primordial follicles (oocyte surrounded by a single layer of flattened pregranulosa cells) using haematoxylin and eosin (H&E) staining. Follicles were scored based on the presence of morphological markers (dense chromatin, eosinophilia, shrunken ooplasm [one point each] or pyknosis [two points]) as normal (0), affected (1), degenerating (2) or atretic (≥ 3). Scale bar: 10 μm.